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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (H&#252;bner)
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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

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Engineering crop determinacy: CRISPR/Cas based advances in self-pruning gene function and application.

Aswathy Rajan1, Muthurajan Raveendran1, Varanavasiappan Shanmugam1

  • 1Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.

Molecular Biology Reports
|May 14, 2026
PubMed
Summary
This summary is machine-generated.

Crop determinate growth, regulated by SELF-PRUNING (SP) genes, enhances productivity. CRISPR genome editing precisely modifies SP/TFL1 genes for optimized plant architecture, improving harvest efficiency and food security.

Keywords:
SELF-PRUNING geneSP/TFL1 geneCRISPR-Cas9Crop determinacyCrop improvementGenome editing

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High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

Area of Science:

  • Plant genetics and breeding
  • Molecular biology
  • Agricultural science

Background:

  • The transition from indeterminate to determinate growth is crucial for crop improvement, impacting flowering, harvest uniformity, and agricultural efficiency.
  • Mutations in SELF-PRUNING (SP) genes, part of the CETS family, control this vegetative to reproductive phase transition and influence shoot architecture in crops like tomato.
  • Increasing global challenges necessitate engineered plant architectures for enhanced productivity and sustainability.

Purpose of the Study:

  • To review the molecular mechanisms governing plant determinacy, focusing on the role of SP/TFL1 genes.
  • To explore the application of CRISPR-based genome editing for modifying SP/TFL1 homologs to achieve determinate growth.
  • To provide a framework for leveraging CRISPR technology in crop improvement for enhanced agricultural systems.

Main Methods:

  • Consolidation of current understanding of molecular mechanisms controlling plant determinacy.
  • Emphasis on the role of SP/TFL1 genes and their interaction with hormonal pathways (auxin, cytokinin).
  • Integration of insights with recent advances in CRISPR-based editing platforms.

Main Results:

  • SP/TFL1 genes are central regulators of the transition to determinate growth.
  • CRISPR genome editing offers a precise method to modify SP/TFL1 homologs.
  • Successful application of genome editing demonstrated across diverse crop species for targeted growth modification.

Conclusions:

  • CRISPR technology enables targeted modification of SP/TFL1 genes for controlled determinate growth.
  • This approach facilitates synchronized flowering and improved mechanical harvestability in crops.
  • Leveraging CRISPR for crop improvement holds significant potential for enhancing agricultural productivity, resilience, and sustainability.