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Updated: May 16, 2026

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
Published on: August 13, 2019
Linear and Nonlinear Spectroscopic Insights Into the Synergistic Solvation of Rhodamine-6G in
Rahul Kumar Gupta1, Surya Kant2, Debabrata Goswami3,4
1Department of Chemistry, Hari Singh College, Munger University, Haveli Kharagpur, Munger, , Bihar, 811213, India.
None:
This study shows that intermolecular interactions in methanol-dichloromethane (MeOH-DCM) binary solvent mixtures play a crucial role in governing the solvation dynamics of the fluorescent probe rhodamine-6G (R6G). By employing both linear (UV-Vis absorption and steady-state fluorescence) and nonlinear (two-photon-induced fluorescence, TPIF) spectroscopic techniques, we demonstrate that the combined effects of solute-solvent and solvent-solvent interactions significantly modify the local environment around R6G. Methanol, a polar protic solvent, and dichloromethane, a polar aprotic solvent, interact through hydrogen bonding and dipole-dipole interactions, creating a microenvironment with an effective polarity higher than that of either pure solvent. As a result, the absorption spectrum exhibits a noticeable red shift, accompanied by enhanced TPIF intensity and a maximum two-photon excitation action cross-section in the mixed solvent system. These findings highlight that carefully choosing and tailoring the solvent provides a simple yet practical way to effectively tune two-photon absorption behavior. Such an approach offers promising opportunities for applications in high-sensitivity bioimaging, optical materials, and photonic devices, where strong nonlinear optical responses are essential.
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