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Long Noncoding RNA MIR205HG Elevates FSTL1 Expression to Drive Calcium Oxalate Nephrolithiasis in vitro through
Min Zhou1,2, Chuntao Chen2, Zhenhai Zou2
1Yangzhou University Medical College, Yangzhou, China.
Kidney & Blood Pressure Research
|May 14, 2026
Summary
Long noncoding RNA MIR205HG plays a role in calcium oxalate nephrolithiasis by regulating the miR-5581-3p/FSTL1 pathway. This finding offers new insights into kidney stone disease mechanisms.
Area of Science:
- Nephrology
- Molecular Biology
- Genetics
Background:
- Nephrolithiasis (kidney stones) has increasing global prevalence and recurrence rates.
- Long noncoding RNAs (LncRNAs) are implicated in kidney stone progression, but the role of MIR205HG is unclear.
Purpose of the Study:
- Investigate the function of lncRNA MIR205HG in calcium oxalate nephrolithiasis.
- Elucidate the competitive endogenous RNA (ceRNA) regulatory network involving MIR205HG.
Main Methods:
- Analyzed MIR205HG expression in patient tissues and cell models (GEO dataset, RT-qPCR).
- Assessed cell viability, apoptosis, oxidative stress, and epithelial-mesenchymal transition (EMT) markers.
- Determined targeting relationships using bioinformatics and luciferase reporter assays.
Main Results:
- MIR205HG expression was upregulated in kidney stone tissues and cells.
- MIR205HG silencing improved cell viability, reduced apoptosis, and mitigated oxidative stress.
- MIR205HG acts as a sponge for miR-5581-3p, which targets FSTL1, influencing cell morphology, EMT, and adhesion.
Conclusions:
- lncRNA MIR205HG is involved in calcium oxalate nephrolithiasis pathogenesis.
- The miR-5581-3p/FSTL1 pathway is a key mechanism regulated by MIR205HG.
- MIR205HG represents a potential therapeutic target for kidney stones.
