Related Experiment Video
Updated: May 16, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Glyceroneogenesis has a new PEP in its step(s)
Alexandra E Jerrett1, Nicolai R Hathiramani2, Jessica B Spinelli3
1Morningside Graduate School of Biomedical Sciences, UMass Chan Medical School, Worcester, MA 01605, USA; Program in Molecular Medicine, UMass Chan Medical School, Worcester, MA 01605, USA.
Abstract:
Mitochondria generate phosphoenolpyruvate (PEP), although its export mechanism and physiological roles were unknown. In this issue of Cell, Kajimura and colleagues identify SLC25A35 as the mitochondrial PEP exporter and uncover a previously unrecognized role for mitochondrial PEP synthesis in glyceroneogenesis in adipose tissue and upon development of fatty liver disease.
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