Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Archaeal Cell Wall01:29

Archaeal Cell Wall

Archaeal cell walls are structurally and compositionally distinct from their bacterial counterparts, lacking the characteristic peptidoglycan layer found in most bacteria. Instead, archaeal cell walls exhibit remarkable diversity, utilizing materials such as pseudomurein, polysaccharides, and proteins to construct their protective outer layers. This structural flexibility is closely tied to archaea's ecological adaptability.S-Layers: The Common Archaeal Cell WallThe S-layer is the most...
Plant Cell Wall02:43

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
Plant Cell Wall01:07

Plant Cell Wall

Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
Bacterial Cell Wall01:22

Bacterial Cell Wall

The bacterial cell wall is an essential structural component that encases the plasma membrane, preserving cellular integrity, determining shape, and protecting against osmotic stress. This rigid yet flexible structure primarily comprises peptidoglycan, a polymer that forms a mesh-like matrix conferring mechanical strength and flexibility.Peptidoglycan Composition and StructurePeptidoglycan, the core of the bacterial cell wall, comprises alternating units of N-acetylglucosamine (NAG) and...
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of disassembly and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rethinking Suicide Thi4 Thiazole Synthases: Comparative Genomic Insights and Pilot Functional Evidence.

ACS omega·2026
Same author

BOTany methods: accessible automation for plant synthetic biology.

Plant physiology·2026
Same author

Continuous Directed Evolution of a Short-Lived Plant Histidinol Dehydrogenase.

ACS synthetic biology·2026
Same author

Golgi-localized mannanases sustain hemicellulose biosynthesis.

The New phytologist·2026
Same author

BGC-MAC and BGC-MAP: Attention-Based Models for Biosynthetic Gene Cluster Classification and Product Matching.

Journal of chemical information and modeling·2025
Same author

Illuminating Glucomannan Synthases To Explore Cell Wall Synthesis Bottlenecks.

ACS synthetic biology·2025
Same journal

Novel Imaging Approaches for Visualising Root-Mycorrhizal Fungal Interactions.

Journal of experimental botany·2026
Same journal

The ga3ox1b mutation reveals the crosstalk between gibberellin and other phytohormones in controlling the growth and development of female flowers in Cucurbita pepo.

Journal of experimental botany·2026
Same journal

Increased grain weight conferred by GW2 mutations in wheat does not translate into yield gains in multi-year field trials of near-isogenic lines.

Journal of experimental botany·2026
Same journal

Serendipita indica promotes rice phosphorus uptake by plasma membrane H+-ATPase OsA1-stimulated root hair growth.

Journal of experimental botany·2026
Same journal

The primary beta-galactosidase BGAL10 modulates pavement cell shape acquisition in Arabidopsis.

Journal of experimental botany·2026
Same journal

The link between phosphate starvation-triggered anthocyanin biosynthesis and jasmonate-driven regulation in tomato.

Journal of experimental botany·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

Elucidating β-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia Infestation
10:26

Elucidating β-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia Infestation

Published on: July 26, 2024

Understanding Cell Wall Enzyme Function: From Classical Approaches to New Biotechnology.

Mair Edwards1, Moni Qiande2, Alan D Gomez Vargas2

  • 1Plant Molecular and Cellular Biology Program, IFAS, University of Florida, Gainesville, FL.

Journal of Experimental Botany
|May 14, 2026
PubMed
Summary
This summary is machine-generated.

This review details methods for studying plant cell wall enzymes (CAZymes), crucial for plant structure and human health. New tools like AI and CRISPR accelerate understanding and engineering of these enzymes for bio-applications.

Keywords:
Artificial intelligenceBiotechnologyGlycosyl hydrolasesGlycosyltransferasesHeterologous hostsPlant cell wallsPolysaccharides

More Related Videos

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

Published on: March 24, 2016

Related Experiment Videos

Last Updated: May 16, 2026

Elucidating β-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia Infestation
10:26

Elucidating β-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia Infestation

Published on: July 26, 2024

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

Published on: March 24, 2016

Area of Science:

  • Plant Biology
  • Biochemistry
  • Biotechnology

Background:

  • Plant cell walls are vital for structure and nutrition, composed of polysaccharides.
  • Carbohydrate-active enzymes (CAZymes) assemble and remodel these cell walls.
  • Characterizing plant CAZymes is challenging due to technical hurdles and genetic redundancy.

Purpose of the Study:

  • To provide a primer on strategies for functional insights into plant cell wall-related CAZymes.
  • To highlight the importance of CAZymes for plant biology and applications in food, bioenergy, and bioproducts.
  • To review classical and emerging methods for CAZyme characterization and engineering.

Main Methods:

  • Classical biochemical assays and genetic approaches.
  • Emerging solutions: high-throughput screening, cell-free systems, microbial and plant-based expression platforms.
  • Synthetic biology tools: CRISPR/Cas9 genome editing, base editing, modular expression systems, AI protein models, and lab automation.

Main Results:

  • Functional insights into CAZymes are essential for advancing plant biology.
  • New technologies accelerate the functional annotation and engineering of CAZymes.
  • Integrative approaches offer efficient design of plant cell walls for improved nutrition and sustainable bioproducts.

Conclusions:

  • Understanding plant CAZymes is key to unlocking their potential in various industries.
  • Synthetic biology and AI integration promise efficient engineering of cell wall components.
  • This research facilitates the development of improved nutrition and sustainable bioproducts within a circular bioeconomy.