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Related Experiment Video

Updated: May 16, 2026

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Integrated single-cell and spatial transcriptomic profiling in ALS uncovers peripheral-to-central immune infiltration

Ziyang Zhang1,2, Lynn van Olst1,2, Francesco Alessandrini2

  • 1Abrams Research Center on Neurogenomics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Nature Neuroscience
|May 14, 2026
PubMed
Summary

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This summary is machine-generated.

This study reveals immune system changes in amyotrophic lateral sclerosis (ALS), linking peripheral and central immune alterations to disease subtypes and progression. Findings suggest targeted immunomodulation may be a future therapeutic strategy for ALS patients.

Area of Science:

  • Neuroscience
  • Immunology
  • Genetics

Background:

  • Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by motor neuron degeneration.
  • Neuroinflammation is a key feature of ALS, but its molecular links to motor neuron pathology are unclear.

Purpose of the Study:

  • To investigate shared and distinct immune dynamics in sporadic ALS and C9orf72-associated ALS.
  • To identify molecular programs connecting immune responses to motor neuron degeneration.

Main Methods:

  • Integrated analysis of single-cell RNA sequencing, bulk RNA sequencing, and spatial proteogenomics.
  • Characterization of immune cells in peripheral blood and spinal cord tissues from ALS patients.

Main Results:

Related Experiment Videos

Last Updated: May 16, 2026

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

  • Identified broad immune remodeling in C9orf72 ALS.
  • Revealed ALS subtype-specific and progression-associated differences in monocyte and CD8 T cell responses.
  • Spatial mapping showed complement activation and myeloid states at sites of motor neuron loss and TDP-43 pathology.

Conclusions:

  • Connected peripheral and central immune alterations to ALS heterogeneity.
  • Highlighted stratified immunomodulation as a potential therapeutic strategy for ALS.