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Updated: May 16, 2026

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
The SPL transcription factor OsSPL10 regulates rice tillering through auxin signaling to maintain grain yield under
Yingxiu Li1, Wei Ye1, Haifeng Guo1
1Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Abstract:
Maintenance of high, stable yields under environmental stress has been a long-standing objective in rice breeding. Here, we demonstrate that the SPL transcription factor OsSPL10 has a crucial role in the regulation of tillering and grain yield under both normal and drought-stress conditions. Haplotype analysis showed that OsSPL10T-Hap1 is a favorable allele associated with increased tiller number and enhanced drought tolerance, and rice varieties carrying OsSPL10T-Hap1 produce more effective tillers and have significantly higher grain yields than those carrying OsSPL10T-Hap2. Further experiments revealed that OsSPL10 directly regulates the expression of the Aux/IAA gene OsIAA13 to modulate tillering through the auxin signaling pathway. Genome editing of OsSPL10 in the Shennong265 background led to a 14.22% increase in grain yield under field conditions, and the edited plants also showed increases in effective tiller number and grain yield under drought-stress conditions. These findings highlight OsSPL10 as a potential breeding target for the development of rice varieties with high and stable yields.
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