Related Experiment Video
Updated: May 16, 2026

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
Published on: July 28, 2022
Hyaluronidase Reverses the Effect of Hyaluronic Acid Without Destroying Normal Skin Structure
Bin Yin1, Zhiling Jiang1, Jian Shi2
1The First Affiliated Hospital, Center of Burn & Plastic and Wound Healing Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China.
Background:
Dermal fillings based on hyaluronic acid have been widely used in facial rejuvenation. A number of studies have reported the beneficial effect of hyaluronidase in saving the complications of hyaluronic acid. However, the effects of hyaluronidase on dermal matrix, skin structure, and fibroblast function are unknown.
Method:
In this study, hyaluronic acid and hyaluronidase were injected into rat skin, skin samples were collected at multiple time points, and the changes of skin structure, fibroblast function, and extracellular matrix gene and protein expression were detected.
Result:
The results in vivo showed that hyaluronidase can effectively degrade exogenously injected hyaluronic acid in rats without causing significant changes in dermal matrix and skin structure. The results in vitro showed that hyaluronidase had no significant effect on proliferation, apoptosis, and collagen synthesis of fibroblasts.
Conclusion:
This study provided a theoretical basis for the clinical application of hyaluronic acid and hyaluronidase. In vivo and in vitro experiments confirmed that hyaluronidase can degrade exogenous hyaluronic acid without damaging the skin structure, dermal matrix, and fibroblast function.
Related Concept Videos
Glycosaminoglycans
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
Hydrolysis
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Clinical Applications of Epidermal Stem Cells
Lysosomal Hydrolases
Tissue Renewal without Stem Cells
However, failure of such a system...
Renewal of Skin Epidermal Stem Cells

