Related Experiment Video
Updated: May 17, 2026

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
Published on: July 30, 2020
In Vivo Absorption and Tissue Distribution of Nanocapsulated Roselle Anthocyanins under Fasted and Fed States
Nageena Qayyum1, Wanhan Song1, Ya Wang1
1Department of Nutrition, School of Public Health, Guangdong Medical University, Dongguan 523808, China.
Abstract:
This study investigated the absorption and tissue distribution of nanocapsulated roselle anthocyanin extract (Nano-RAE) in C57BL/6 mice under both fasted and fed conditions. Following gavage, Nano-RAE delayed the appearance of cyanidin-3-glucoside (C3G) and delphinidin-3-glucoside (D3G) in the liver, accompanied by enhanced metabolite formation and sustained concentration profiles consistent with controlled release. In contrast, unencapsulated roselle anthocyanin extract exhibited rapid tissue distribution, with higher brain levels of C3G and greater renal concentrations of pelargonidin-, petunidin-, and peonidin-3-glucoside. Additionally, Nano-RAE resulted in lower hepatic protocatechuic acid levels. Although anthocyanins were rapidly metabolized, intact C3G and D3G were detected in the brain, indicating penetration of the blood-brain barrier. Molecular docking revealed strong binding of C3G to mouse multidrug resistance protein 1a, suggesting an interaction that may affect its bioavailability and tissue distribution. These findings highlight the influence of encapsulation and feeding state on anthocyanin absorption and provide insights for designing dietary interventions.
Related Concept Videos
Modified-Release Drug Delivery Systems: Bioavailability
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

