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Updated: May 17, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin
Yutaka Ouchi1, Koki Ishii1, Yumiko Sato1
1Department of Applied Chemistry, Kindai University, Higashiosaka, Japan.
Abstract:
The development of methods for detecting specific nucleic acids is important for early diagnosis and treatment of diseases at the genetic level. We have developed a pair of pyrene (Pyr)-modified peptide nucleic acids (PNAs), PNA twin probe, as a tool for such detection. In this study, we prepared Pyr-PNAs containing chloroacetyl (-COCH2Cl) or thiol (-SH) groups at the termini by solid-phase peptide synthesis. By analyzing various candidates, we clarified that a pair of Pyr-PNAs, each containing an SH group, formed a disulfide bond through the hybrid formation of two PNAs with complementary DNA, resulting in excimer emission at 455 nm. Furthermore, we demonstrated that these Pyr-PNAs provide fluorescent detection of intracellular target RNAs through enhanced excimer emission via the ligation. This work should aid future studies aimed at the specific fluorescent detection of RNA in living cells.
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