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The SNARE Protein RpUSE1 Is Essential for Female Reproduction and Organelle Homeostasis in Riptortus pedestris.

Yan-Yan Yang¹, Ying Tian¹, Qing-Kai Feng¹

¹State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.

Archives of Insect Biochemistry and Physiology

|May 15, 2026

Summary

The SNARE protein RpUSE1 is vital for insect survival and reproduction. Silencing this gene in Riptortus pedestris pests disrupts nymph development and female fertility, impacting organelle structure and transport.

Keywords:

Riptortus pedestris RpUSE1 SNARE lateral oviduct reproduction traffic

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Area of Science:

Molecular Biology
Cell Biology
Entomology

Background:

The SNARE protein USE1 is crucial for retrograde membrane traffic in yeast, but its role in insects is unknown.
This study focuses on the RpUSE1 gene in the agricultural pest Riptortus pedestris.

Purpose of the Study:

To investigate the function of the RpUSE1 gene in Riptortus pedestris.
To determine the role of RpUSE1 in nymphal development and adult reproductive success.

Main Methods:

Domain analysis of the RpUSE1 protein.
RNA interference (RNAi)-mediated knockdown of RpUSE1 in R. pedestris.
Analysis of nymph survival, development, and female reproductive parameters.

Main Results:

RpUSE1 is a ubiquitously expressed SNARE protein with the highest levels in the female reproductive system.
RpUSE1 knockdown severely impaired nymph survival and development.
Silencing RpUSE1 in females disrupted egg development, caused oviduct structural disorder, and ER disorganization, with lipid droplet accumulation.

Conclusions:

RpUSE1 is essential for nymphal survival and female reproductive success in R. pedestris.
The protein plays a critical role in maintaining organelle structure and intracellular transport, particularly in the female reproductive system.