Related Experiment Video
Updated: May 17, 2026
06:51
Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
Published on: December 17, 2019
Protocol for a multi-modal performance-profiling workflow of CAR T cell products
Sarah Schulenberg1, Michelle Loeser1, Michael Schmueck-Henneresse2
1Berlin Institute of Health (BIH) Center for Regenerative Therapies, Experimental Immunotherapy, BIH at Charité - Universitätsmedizin Berlin, Berlin 13353, Germany.
STAR Protocols
|May 15, 2026
Summary
This study introduces a multi-modal profiling method for engineered T cell products, combining flow cytometry and tumor-killing assays. This approach enhances the functional characterization of CAR T cells for adoptive cell therapy development.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Functional characterization is crucial for developing effective adoptive cell therapies.
- Current methods may not fully capture the complex behavior of engineered T cells.
Purpose of the Study:
- To present a comprehensive protocol for multi-modal profiling of engineered T cell products.
- To enable detailed functional characterization of Chimeric Antigen Receptor (CAR) T cells.
Main Methods:
- Utilized 33-marker full-spectrum flow cytometry (FSFC) for detailed cell surface marker analysis.
- Integrated FSFC with live-cell serial tumor-killing imaging to assess cytotoxic function.
- Described procedures for cell preparation, antibody staining, data acquisition, and analysis.
Main Results:
- Demonstrated a combined approach for robust multi-modal profiling of CAR T cell products.
- Provided a detailed protocol adaptable for various engineered T cell therapies.
- Enabled comprehensive assessment of T cell function beyond traditional assays.
Conclusions:
- The presented multi-modal profiling protocol offers a powerful tool for characterizing engineered T cells.
- This method is essential for advancing the development and clinical application of adoptive cell therapies.
- The protocol's adaptability supports its broad utility in immunotherapy research.