Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Faculty Mentor Development Intervention on Mentees' Subjective Career Success.

The chronicle of mentoring & coaching·2026
Same author

Impact of Mentors on Overall Faculty Experience and Reason for Leaving.

The chronicle of mentoring & coaching·2026
Same author

Effectiveness of a Structured Curriculum-Based Developmental Network Intervention.

The chronicle of mentoring & coaching·2026
Same author

Abstracts of the 26th International Workshop on Clinical Pharmacology of HIV, Hepatitis and other Antiviral Drugs 2025, 3-4 September 2025, Amsterdam, the Netherlands.

British journal of clinical pharmacology·2025
Same author

Differences in neurophysiological resting-state alpha spectral events in 4-12-year-old children with prenatal exposure to alcohol relative to typically developing controls.

Neuroscience·2025
Same author

Reasons for Faculty Attrition, Assessed by Latent Class Analysis.

The chronicle of mentoring & coaching·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: May 17, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Mid-infrared quantum cascade laser-based structured illumination microscope.

M Le, O Myers, J Squier

    Optics Letters
    |May 15, 2026
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new mid-infrared microscopy technique using Spatial Frequency Modulated Imaging (SPIFI) and a Quantum Cascade Laser (QCL). This cost-effective method enables fast, chemically specific imaging, overcoming limitations of current mid-infrared microscopes.

    More Related Videos

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    Related Experiment Videos

    Last Updated: May 17, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Spectroscopy

    Background:

    • Mid-infrared (3-20 μm) hyperspectral imaging offers unique molecular contrast for chemical mapping.
    • Traditional mid-infrared microscopes face challenges with slow acquisition speeds and high costs.
    • Existing methods often lack the speed and cost-effectiveness for widespread adoption.

    Purpose of the Study:

    • To demonstrate the first implementation of Spatial Frequency Modulated Imaging (SPIFI) in the mid-infrared region.
    • To develop a cost-effective and faster hyperspectral imaging technique for mid-infrared microscopy.
    • To overcome the limitations of conventional mid-infrared microscopy regarding speed and detector costs.

    Main Methods:

    • Utilized a Quantum Cascade Laser (QCL) as the light source.
    • Employed spatial frequency-encoded structured illumination.
    • Integrated a low-cost single-pixel mid-infrared detector for data acquisition in transflection mode.

    Main Results:

    • Achieved hyperspectral imaging in the mid-infrared with a practical trade-off between speed and complexity.
    • Demonstrated acquisition speeds comparable to some QCL-based point-scanning systems.
    • Presented preliminary resolution measurements and absorption images of mouse brain coronal sections at 6 and 7 μm.

    Conclusions:

    • The developed SPIFI microscopy system offers a cost-effective and efficient solution for mid-infrared hyperspectral imaging.
    • The technique provides chemically selective contrast and robust image formation.
    • The system is suitable for future advancements in high-resolution and compressive imaging applications.