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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

IFN-γ Engineering Renders Tumor Cells Autonomous and Bystander Tumoricidal Activities.

Haoyang Li1, Yunfei Zhu1, Yan Hao1

  • 1State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Cancer Biology Center, Frontiers Science Center for New Organic Matter, College of Life Sciences, Nankai University, Tianjin, 300071, China.

Biochemical and Biophysical Research Communications
|May 15, 2026
PubMed
Summary
This summary is machine-generated.

Scientists reprogrammed tumor cells to express T cell effectors. Engineered cells inhibited tumor growth and killed neighboring cancer cells, with Interferon-gamma (IFNG) showing the strongest effect, offering a novel cancer therapy strategy.

Keywords:
Fas ligandGranzyme BIFN-γPerforinTumor reprogramming

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Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Published on: January 14, 2011

Area of Science:

  • Oncology
  • Immunotherapy
  • Cancer Biology

Background:

  • Cancer is a significant global health issue.
  • Tumor reprogramming is a promising therapeutic strategy.
  • The ability to stably reprogram tumor cells into cytotoxic effectors is not well understood.

Purpose of the Study:

  • To engineer tumor cells to express T cell effector genes (IFNG, FASLG, PRF1/GZMB).
  • To evaluate the cytotoxic potential and therapeutic efficacy of these reprogrammed tumor cells.

Main Methods:

  • Engineering multiple tumor cell types to overexpress specific T cell effector genes.
  • In vitro assessment of cell autonomous proliferation inhibition.
  • Co-culture assays to evaluate tumor cell-mediated cytotoxicity.
  • In vivo studies to assess tumor growth suppression and toxicity.

Main Results:

  • Engineered tumor cells showed inhibited proliferation in vitro.
  • Overexpression of IFNG or FASLG led to tumor cells killing neighboring tumor cells.
  • IFNG-mediated cytotoxicity was significantly stronger than FASLG-mediated cytotoxicity.
  • PRF1/GZMB overexpression did not result in tumor cell killing.
  • IFNG-overexpressing tumor cells suppressed wild-type tumor growth in vivo with no apparent toxicity.

Conclusions:

  • Tumor cells can be reprogrammed to acquire tumor-killing activity.
  • IFNG overexpression in tumor cells is a potent strategy for inducing cytotoxicity.
  • This approach presents a potential new direction for cancer therapies.