The diagnostic significance of lncRNA BANCR in Glaucoma

Primary open-angle glaucoma (POAG) is a chronic and progressive condition affecting the optic nerve. This study aimed to evaluate the diagnostic significance of lncRNA BANCR in POAG. lncRNA BANCR and miR-339-5p levels in the serum of the research objects and in HTMCs were detected using qRT-PCR. The diagnostic performance of BANCR for POAG was evaluated through ROC curve. Logistic analysis was used to investigate independent risk factors for POAG. Cell viability and apoptosis were detected using CCK8 reagent and flow cytometry. The levels of SOD and MDA were detected by test kit. BANCR and miR-339-5p showed correlated expression patterns as determined by Pearson's r analysis. BANCR was upregulated in POAG patients and had high diagnostic value. In addition, BANCR is an independent risk factor for POAG. H₂O₂ treatment increased BANCR levels, whereas silencing BANCR decreased the levels. In addition, H₂O₂ treatment reduced cell viability, accelerated apoptosis, and induced oxidative stress, whereas silencing of BANCR reversed these effects. miR-339-5p and BANCR were negatively correlated in the serum of POAG patients. Simultaneous silencing of BANCR and miR-339-5p reversed the effects of silencing BANCR alone on cell viability, apoptosis, and oxidative stress. BANCR may be a diagnostic biomarker for POAG. Additionally, BANCR may regulate POAG progression by targeting miR-339-5p.