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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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  3. The Competition Between Splicing And 3' Processing Shapes The Human Transcriptome.
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  3. The Competition Between Splicing And 3' Processing Shapes The Human Transcriptome.

Related Experiment Video

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

The competition between splicing and 3' processing shapes the human transcriptome.

Lindsey V Soles1,2, Shuangyu Li1, Liang Liu1

  • 1Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, California 92617, USA.

Genes & Development
|May 15, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Splicing and 3' processing are linked. Disrupting splicing activates intronic polyadenylation (IPA) sites and premature transcription termination, revealing competition between these RNA processing steps.

Keywords:
cleavage and polyadenylationgene expressionintronic polyadenylationpre-mRNA 3′ processingpre-mRNA processingpre-mRNA splicingtranscription elongationtranscription regulation

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Related Experiment Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Molecular Biology
  • RNA Processing
  • Gene Regulation

Background:

  • Eukaryotic pre-mRNA processing involves coordinated splicing and 3' processing.
  • The proposed telescripting model suggests U1 snRNP inhibits 3' processing at intronic polyadenylation (IPA) sites.
  • Mechanisms coordinating these processes remain unclear.

Purpose of the Study:

  • To investigate the relationship between splicing and 3' processing.
  • To determine the role of splicing factors in regulating IPA site usage.
  • To elucidate how splicing and 3' processing influence transcription.

Main Methods:

  • Disruption of splicing using six distinct methods targeting key spliceosome components (U1 snRNP, U2 snRNP, U2AF, SF3b).
  • Analysis of intronic polyadenylation (IPA) site activation.
  • Assessment of premature transcription termination.
  • Global analysis of splicing and 3' processing upon inhibition of the other.

    • Main Results:

    • Splicing inhibition activated thousands of IPA sites.
    • Splicing inhibition, particularly of U1 snRNP, caused widespread premature transcription termination.
    • Inhibition of 3' processing globally enhanced splicing.
    • Distinct splicing factors influenced overlapping and unique sets of IPA sites.

    Conclusions:

    • Splicing and 3' processing are competing processes.
    • These processes intersect with transcription to shape the transcriptome.
    • The findings challenge the telescripting model and propose a competitive model for RNA processing and transcription regulation.