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Published on: July 19, 2016
Remineralization and antimicrobial bioactivities of a Sn-CPP-ACFP nanocomplex
Peiyan Shen1, James R Fernando1, Yi Yuan1
1Centre for Oral Health Research, Melbourne Dental School, The University of Melbourne, Melbourne, Victoria 3010, Australia.
Summary
The novel tin-casein phosphopeptide-amorphous calcium fluoride phosphate (Sn-CPP-ACFP) nanocomplex significantly enhanced enamel and dentine remineralisation and inhibited oral bacteria. This new nanocomplex offers superior antimicrobial and remineralisation effects compared to traditional fluoride treatments.
Area of Science:
- Biomaterials Science
- Dental Research
- Nanotechnology
Background:
- Dental caries remains a significant global health issue.
- Remineralisation therapies are crucial for preventing and reversing early enamel demineralisation.
- Antimicrobial agents are needed to combat cariogenic bacteria like Streptococcus mutans and Porphyromonas gingivalis.
Purpose of the Study:
- To evaluate the remineralisation potential of a novel tin-CPP-ACFP nanocomplex.
- To assess the antimicrobial bioactivities of the Sn-CPP-ACFP nanocomplex against key oral pathogens.
- To compare the efficacy of Sn-CPP-ACFP with traditional stannous fluoride (SnF2) rinses.
Main Methods:
- Sn-CPP-ACFP nanocomplex was synthesized using CPP-ACP and SnF2.
- An in situ human enamel and dentine remineralisation assay was conducted over 14 days.
- Participants rinsed with Sn-CPP-ACFP (CPPSF) or SnF2 (SF) solutions.
- Remineralisation was assessed via microhardness recovery (%SMHR) and transverse microradiography.
- Antimicrobial activity was tested against Streptococcus mutans and Porphyromonas gingivalis using agar diffusion.
Main Results:
- CPPSF demonstrated superior inhibition of Streptococcus mutans and Porphyromonas gingivalis growth.
- Significantly higher enamel remineralisation (35.9%) and dentine remineralisation (37.9%) were observed with CPPSF compared to SF.
- %SMHR was markedly higher for CPPSF (38.2%) versus SF (4.7%).
- CPPSF treatment led to increased bioavailable calcium and tin ions in saliva and enhanced dentine tubule occlusion.
Conclusions:
- The novel Sn-CPP-ACFP nanocomplex exhibits enhanced remineralisation capabilities for both enamel and dentine.
- Sn-CPP-ACFP possesses significant antimicrobial activity against cariogenic bacteria.
- This nanocomplex represents a promising advancement over traditional fluoride treatments for caries management.
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