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Dual-Electrode Wearable Biosensors for In-Field MicroRNA Analysis in Living Plants.

Xiaojuan Liu1, Jiahui Zhao2, Yang Xue2

  • 1College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, P. R. China.

Analytical Chemistry
|May 15, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a wearable biosensor for detecting plant microRNAs (miRNAs) directly in the field. This innovation advances precision agriculture by enabling sensitive, real-time monitoring of plant stress responses.

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Area of Science:

  • Plant molecular biology
  • Biosensor technology
  • Agricultural science

Background:

  • MicroRNAs (miRNAs) are key regulators of plant growth and stress responses.
  • Monitoring miRNA expression in living plants is challenging due to low abundance and complex sample matrices.
  • Plant wearable sensors offer potential for advancing plant physiology studies and precision agriculture.

Purpose of the Study:

  • To design and fabricate a plant wearable dual-electrode ratiometric chip (DER-Chip) for sensitive, direct, in-field miRNA determination.
  • To overcome limitations of current miRNA detection methods in complex plant tissues.
  • To enable real-time assessment of miRNA levels in plants under stress.

Main Methods:

  • Fabrication of a flexible DER-Chip with a hydrogel electrode and a gold nanoparticle-modified laser-induced graphene (LIG/Au) electrode.
  • Utilized target-triggered hybridization chain reaction amplification for signal enhancement.
  • Implemented a ratiometric electrochemical signal output and a magnetic-driven directional transfer strategy.
  • Demonstrated direct miRNA determination without target enrichment or pretreatment.

Main Results:

  • The DER-Chip achieved highly sensitive detection of plant miRNAs, with a limit of detection as low as 3.2 fM.
  • The sensor demonstrated excellent analytical performance in complex plant extrudates.
  • The system is suitable for direct, in-field monitoring of miRNA expression.

Conclusions:

  • The developed wearable DER-Chip provides a sensitive and direct method for plant miRNA detection in field conditions.
  • This technology has significant implications for understanding plant physiology and advancing precision agriculture.
  • The chip can be integrated with portable analyzers for in situ assessment of plant stress responses.