Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 17, 2026

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

Spatial transcriptomic profiling of developing mouse hearts reveals a spatially patterned signaling environment.

Junqi Hu^1,2, Haoting He¹, Juan Xu¹

¹Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Communications Biology

|May 15, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Automated Radiosynthesis of High-Molar-Activity 4-Borono-2-[<sup>18</sup>F]Fluorophenylalanine: Validation in Rodent Tumor Models and Nonhuman Primates.

Molecular pharmaceutics·2026

Same author

CausalTCC: causal temporal contrastive learning for automated Alzheimer's disease biomarker discovery with bio-electrical signals.

Journal of neural engineering·2026

Same author

Macrophage-fibroblast signaling networks identified by single-cell RNA sequencing in juvenile systemic sclerosis.

JCI insight·2026

Same author

eQTM (expression quantitative trait methylation) Atlas: a comprehensive resource of over 11 million DNA methylation-gene expression associations through across 11 tissues and 4 diseases.

bioRxiv : the preprint server for biology·2026

Same author

Case Report: Adult-onset Still's disease presenting with massive hemorrhagic pericardial effusion without the characteristic rash.

Frontiers in medicine·2026

Same author

Reframing Partial Root-Zone Irrigation: A Spatial Stress-Priming Mechanism for Crop Adaptation to Abiotic Stresses.

Plants (Basel, Switzerland)·2026

This study maps developing mouse hearts using spatial transcriptomics, revealing niche signaling crucial for cardiac conduction cells and epicardial cell-derived cells (EPDCs). Ablating EPDCs reduced signaling, highlighting their role in heart development.

Area of Science:

Developmental Biology
Cardiovascular Research
Genomics

Background:

Heart development relies on intricate cell-niche signaling.
Single-cell mRNA sequencing (scRNA-seq) struggles to capture spatial context.
Spatial transcriptomics offers a solution by preserving tissue architecture.

Purpose of the Study:

To create a spatial transcriptomic atlas of developing mouse hearts.
To investigate niche signaling in cardiac development.
To identify cell types and their interactions within the developing heart.

Main Methods:

Utilized 10x Genomics Visium and Curio Slide-seq platforms for spatial transcriptomics.
Analyzed spatial gene expression patterns, cell cycle, and myocardial signatures.

More Related Videos

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Published on: May 24, 2024

Related Experiment Videos

Last Updated: May 17, 2026

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Published on: May 24, 2024

Integrated lineage tracing with spatial transcriptomics and employed cell ablation.

Main Results:

Generated a spatial atlas of embryonic and neonatal mouse hearts.
Identified spatial patterns of cell cycle, myocardium types, and chamber-specific genes.
Discovered mature atrial cardiomyocyte signatures, cardiac conduction cells, and four EPDC types with their niche signaling.

Conclusions:

Spatial transcriptomics provides key insights into heart development and niche signaling.
Cardiac conduction cells and EPDCs play significant roles in heart development.
EPDC ablation impacts signaling in developing hearts.