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Lymphoid Cells and Tissues

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...

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Updated: May 18, 2026

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Two-Trace Two-Dimensional (2T2D) Correlation Spectroscopy of Lymphocytes in Infectious Mononucleosis.

Natalia Stach¹, Aleksandra Kurek¹, Magdalena Pietruszewska¹

¹Faculty of Chemistry, Jagiellonian University, Kraków, Poland.

Applied Spectroscopy

|May 16, 2026

Summary

This summary is machine-generated.

Raman spectroscopy can detect early-stage Epstein-Barr virus (EBV) infection in lymphocytes. This technique identifies biochemical changes, showing potential as a non-invasive diagnostic tool for viral infections.

Keywords:

2T2D EBV Epstein–Barr virus Infectious mononucleosis Raman spectroscopy lymphocytes two-trace two-dimensional correlation spectroscopy

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Published on: December 17, 2015

Area of Science:

Biophysics
Biochemistry
Spectroscopy

Background:

Raman spectroscopy is effective for analyzing biological macromolecules.
Epstein-Barr virus (EBV, HHV-4) is a widespread virus causing infectious mononucleosis.
Early detection of EBV infection in lymphocytes is crucial.

Purpose of the Study:

To assess Raman spectroscopy's ability to differentiate healthy lymphocytes from those infected with EBV at an early stage.
To identify specific biochemical alterations indicative of EBV infection.

Main Methods:

Collected blood samples from infectious mononucleosis patients and healthy volunteers.
Acquired Raman spectra using 514.5 nm and 785 nm excitation wavelengths.
Applied the two-trace two-dimensional (2T2D) correlation method for comparative analysis.

Main Results:

Distinct differences were observed between healthy and EBV-infected lymphocytes using asynchronous correlation spectra.
Specific biochemical changes, including immunoglobulin formation (514.5 nm) and Tyrosine phosphorylation (785 nm), were identified.
These changes correlate with lymphocyte activation and EBV infection.

Conclusions:

Raman spectroscopy can distinguish between healthy and EBV-infected lymphocytes.
The technique shows promise as a non-invasive diagnostic tool for early viral infection detection.
Biochemical alterations detected by Raman spectroscopy provide insights into EBV's impact on lymphocytes.