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Related Concept Videos

Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Biological and Biomedical Applications of Optical Photothermal Infrared Spectroscopy (O-PTIR).

Twinkle Soni1,2, Diana E Bedolla1,3, Bayden R Wood1,2

  • 1Centre for Biospectroscopy, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.

Applied Spectroscopy
|May 16, 2026
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Summary

Optical photothermal infrared (O-PTIR) spectroscopy offers high-resolution, label-free molecular imaging for biological research. This review details its principles, applications in cells and tissues, and future directions like AI integration.

Keywords:
IR spectroscopyO-PTIROptical photothermal infraredRaman spectroscopyfluorescencesub-micrometer

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Area of Science:

  • Biomedical Engineering
  • Spectroscopy
  • Molecular Imaging

Background:

  • Optical photothermal infrared (O-PTIR) spectroscopy combines infrared chemical specificity with visible-light excitation for high spatial resolution.
  • It builds upon photoacoustic and photothermal infrared methods, offering advantages like sub-micrometer resolution and label-free detection.
  • O-PTIR is compatible with complex biological samples, enabling detailed molecular analysis.

Purpose of the Study:

  • To provide a comprehensive overview of O-PTIR technology.
  • To highlight its expanding applications in biological and biomedical research.
  • To discuss current limitations and future directions.

Main Methods:

  • Review of foundational principles, including the photothermal effect and instrumental configurations.
  • Integration of simultaneous Infrared (IR) and Raman measurements.
  • Analysis of O-PTIR's capability for imaging biomolecules (lipids, proteins, nucleic acids, metabolites).

Main Results:

  • O-PTIR enables high-resolution, chemically specific imaging of biomolecules in cells, tissues, and microbial systems.
  • Applications include cellular metabolism, microbial phenotyping, cancer diagnostics, biomarker identification, and pharmaceutical analysis.
  • Emerging directions include live-cell imaging, AI-driven analysis, and hybrid modalities like FISH-O-PTIR.

Conclusions:

  • O-PTIR is a transformative technology for biological and biomedical sciences.
  • It bridges fundamental molecular studies with clinical and translational applications.
  • Continued innovation promises to expand its impact in diverse research areas.