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Updated: May 18, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
Metalens Enables Parallel Chromatic Confocal Imaging over a Millimeter-Scale Depth Range with an Axial
Yu-Jie Lin1, Linghan Zhao1, Md Tarek Rahman1
1Department of Electrical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.
Abstract:
Confocal microscopy provides optical sectioning and micrometer-scale resolution but remains constrained by mechanical axial scanning and bulky optics. Leveraging the intrinsic chromatic dispersion of a custom-designed metalens, we demonstrate parallel confocal depth imaging over a 1.3 mm axial range within a 200 nm visible bandwidth by encoding wavelengths into different axial positions to eliminate mechanical axial scanning, which yields an axial space-bandwidth product (ASBP) of ≈68. A single-mode fiber functions simultaneously as an illumination/collection probe and confocal pinhole, forming a compact design. The system achieves an axial resolution of 11.4 μm (axial sensitivity of 0.15 μm) and a lateral resolution of 2.19 μm near the optimal wavelength of 530 nm. The parallel confocal imaging capability across an extended depth is further validated by depth-resolved imaging and surface profile measurement. This compact, label-free platform underscores the potential of meta-optical chromatic confocal imaging for large-depth, high-resolution microscopic applications.

