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Updated: May 18, 2026

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
03:53

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Published on: November 10, 2023

Clinical validation of a multiplex colorimetric RT-LAMP assay for simultaneous RSV-A and RSV-B detection in a

Caroline C Cavalcante1, Letícia T Almeida1, Ana Paula M Franco-Luiz1

  • 1Laboratório de Imunologia de Doenças Virais, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.

Diagnostic Microbiology and Infectious Disease
|May 16, 2026
PubMed

Keywords:
Clinical validationMultiplex RT-LAMPPoint-of-care testingReal-world settingRespiratory Syncytial Virus (RSV)

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...