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A hyperspectral imaging framework integrating band selection and deep learning for beverage stain classification in
Jitendra Shit1, Partha Pratim Roy2, V M Manikandan3,4
1Department of Computer Science and Engineering, SRM University-AP, Amaravati, 522240, Andhra Pradesh, India.
Scientific Reports
|May 16, 2026
Summary
Hyperspectral imaging combined with deep learning accurately identifies beverage stains at mock crime scenes. The Multi-Layer Perceptron model achieved 95.58% accuracy for non-destructive forensic analysis.
Area of Science:
- Forensic Science
- Spectroscopy
- Machine Learning
Background:
- Hyperspectral Imaging (HSI) is crucial for non-destructive forensic analysis, detecting subtle spectral variations.
- Beverage stains are common evidence at crime scenes, requiring reliable identification methods.
Purpose of the Study:
- To investigate the potential of HSI and deep learning for identifying nine types of beverage stains.
- To compare the performance of four deep learning architectures for stain classification.
Main Methods:
- Collected hyperspectral images of beverage stains (Papaya, Coffee, Pomegranate, Orange, Tea, Wine, Whisky, Rum, Brandy) in a controlled mock crime scene.
- Utilized ANOVA-based feature selection to reduce spectral redundancy, selecting 162 bands from 204 in the visible and near-infrared range.
- Trained and evaluated four deep learning models: Multi-Layer Perceptron (MLP), 1D-CNN, LSTM, and CNN-LSTM using standardized spectral data and five-fold cross-validation.
Main Results:
- The Multi-Layer Perceptron (MLP) model achieved the highest classification accuracy of 95.58%.
- All four deep learning architectures demonstrated effectiveness in classifying beverage stains based on spectral characteristics.
- Feature selection using ANOVA successfully reduced spectral redundancy while retaining discriminative information.
Conclusions:
- Hyperspectral imaging coupled with deep learning offers a promising non-destructive method for identifying beverage stains in forensic investigations.
- The MLP architecture demonstrated superior performance for this specific classification task.
- This approach has significant potential to enhance forensic crime scene analysis.

