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Diagnostic value of miR-148b-3p in left ventricular hypertrophy patients with hypertension

Kai Xie1, Chunjin Lin2, Yu Wang3,4,5

  • 1Cardiovascular Medicine Department, Guanghan People's Hospital, Deyang, 618300, China.

BMC Cardiovascular Disorders
|May 16, 2026
PubMed

Insights

MicroRNA-148b-3p (miR-148b-3p) is elevated in hypertension with left ventricular hypertrophy (LVH). This microRNA promotes myocardial hypertrophy by targeting KLF4, indicating its potential as a diagnostic marker and therapeutic target.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiology

Background:

  • Left ventricular hypertrophy (LVH) is a common complication of hypertension.
  • Understanding the molecular mechanisms underlying LVH is crucial for developing effective treatments.
  • MicroRNAs (miRNAs) have emerged as important regulators of cardiovascular diseases, including LVH.

Purpose of the Study:

  • To investigate the expression levels of miR-148b-3p in patients with hypertension and LVH.
  • To evaluate the diagnostic value of miR-148b-3p for hypertension-related LVH.
  • To explore the functional role and underlying mechanism of miR-148b-3p in promoting myocardial hypertrophy.

Main Methods:

  • Serum miR-148b-3p levels were quantified using RT-qPCR in 108 hypertensive patients, 105 with hypertension-related LVH, and 112 healthy controls.
  • Receiver operating characteristic (ROC) curve analysis was performed to assess diagnostic accuracy.
  • In vitro (H9c2 cells) and in vivo (Ang II-induced myocardial hypertrophy model) experiments were conducted to elucidate the functional role of miR-148b-3p.

Main Results:

  • Serum miR-148b-3p levels were significantly higher in patients with hypertension and LVH compared to hypertension alone and healthy controls.
  • miR-148b-3p demonstrated good diagnostic performance for hypertension-related LVH (AUC = 0.821).
  • Overexpression of miR-148b-3p promoted myocardial hypertrophy by increasing ANP/BNP levels and reducing cell viability, while its inhibition reversed these effects. Mechanistically, miR-148b-3p was found to directly target KLF4.

Conclusions:

  • miR-148b-3p is significantly upregulated in hypertension with LVH and possesses diagnostic potential.
  • miR-148b-3p promotes myocardial hypertrophy by targeting KLF4, suggesting it is a key regulator of cardiac remodeling in hypertensive heart disease.
  • miR-148b-3p represents a promising novel molecular marker and therapeutic target for hypertension-related LVH.
Abstract

Keywords:
BiomarkerDiagnosisHypertensionLeft ventricular hypertrophymiR-148b-3p

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