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Updated: May 18, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Genomic characterization and comparative analysis of a new Tequatrovirus phage infecting Escherichia coli ST131
Elsa Beurrier1, Floriane Laumay2,3, Juliette Hayer1
1MIVEGEC (Univ. Montpellier, CNRS, IRD), Montpellier, 34394, France.
Objective:
The global rise of antimicrobial resistance with the spread of pathogenic multidrug-resistant clones such as Escherichia coli sequence type (ST) 131 represents a major challenge for public health, renewing interest in bacteriophages as therapeutical agents.
Results:
We report the isolation and characterization of a new bacteriophage, Escherichia phage vB_EcoM-V1EC45, which was isolated from wastewater and was able to infect a clinical E. coli ST131 strain. Its genome is a 170,364 bp double-stranded DNA molecule annotated with 281 coding sequences and 9 tRNAs, including multiple genes involved in antidefense systems. V1EC45 is predicted to harbour a virulent lifestyle. Comparative genomics positioned V1EC45 within the Tequatrovirus genus, and the Tsx outer membrane bacterial protein is predicted to be used as the receptor-binding protein. This work highlights the value of using genomic, structural, and evolutionary analyses to support the directed development of targeted bacteriophage therapeutics.
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