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Related Experiment Video

Updated: May 19, 2026

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
07:44

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

A layer specific histological framework for synaptic quantification in hippocampal CA1.

Mizuho Ebihara1, Sotaro Ichinose1, Mirei Matsumuro1

  • 1Department of Anatomy, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.

Neuroscience Research
|May 17, 2026
PubMed
Summary
This summary is machine-generated.

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This study introduces a new method to quantify synaptic development in the mouse hippocampus, revealing layer-specific maturation and how prenatal valproic acid exposure impacts neural circuits.

Area of Science:

  • Neuroscience
  • Developmental Neuroscience
  • Synaptic Plasticity

Background:

  • Neural circuit maturation relies on coordinated excitatory and inhibitory synapse assembly.
  • Quantitative frameworks for analyzing synaptic development across hippocampal layers and axes are limited.
  • Prenatal valproic acid exposure is a model for autism-associated neurodevelopmental abnormalities and hippocampal disruption.

Purpose of the Study:

  • To develop a quantitative framework for analyzing synaptic development in the mouse hippocampal CA1.
  • To investigate layer- and axis-dependent synaptic maturation patterns.
  • To examine the effects of prenatal valproic acid exposure on synaptic organization in the hippocampal CA1.

Main Methods:

  • Established a layer boundary-based pipeline for wide-field tiled immunohistochemistry.
Keywords:
Computational modelingExcitation–inhibition balanceHippocampal CA1Laminar organizationPrenatal valproic acidSynaptic maturation

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Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording
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Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording

Published on: August 11, 2019

Related Experiment Videos

Last Updated: May 19, 2026

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
07:44

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
11:21

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Published on: November 20, 2018

Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording
14:27

Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording

Published on: August 11, 2019

  • Quantified PSD-95 (excitatory) and gephyrin (inhibitory) puncta across hippocampal CA1 layers (stratum oriens, radiatum, lacunosum-moleculare).
  • Utilized a 2x2 design to compare control and prenatal VPA-exposed mice across dorsal and ventral axes.

    • Main Results:

    • Identified layer-dependent maturation patterns in dorsal CA1, with stratum lacunosum-moleculare showing inverted synaptic trajectories.
    • Prenatal VPA exposure induced layer- and axis-specific alterations in synaptic puncta density, mean area, and total area.
    • A computational model suggested membrane potential fluctuations as a readout for co-varying synaptic components.

    Conclusions:

    • The developed pipeline provides a practical foundation for integrated histological, functional, and developmental analyses of hippocampal CA1 circuits.
    • Synaptic architecture exhibits nonuniform vulnerability to prenatal VPA exposure.
    • Understanding layer- and axis-specific synaptic development is crucial for studying neurodevelopmental disorders.