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Related Experiment Video

Updated: May 19, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
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Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Visualizing Biomolecules within Living Microglia in Complex Environments Using a Clickable Small Fluorogenic

Wonju Kim1, Yeongran Hwang1, Xiao Liu2

  • 1Neural Circuit Research Group, Korea Brain Research Institute, 61, Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.

ACS Chemical Neuroscience
|May 18, 2026
PubMed
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This summary is machine-generated.

A new fluorescent probe, CDr20-CO1, allows real-time visualization of microglial biomolecules. It reveals how the cellular microenvironment influences microglial responses to inflammation, crucial for understanding neurodegenerative diseases.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Microglia are crucial in neurodegenerative diseases, but visualizing their dynamic responses is challenging.
  • Existing tools lack specificity for live imaging in complex brain environments.
  • Understanding microglial organelle and biomolecule dynamics is key for therapeutic development.

Purpose of the Study:

  • To develop a novel dual-functional fluorescent probe for live-cell imaging of microglial biomolecules.
  • To investigate the influence of the cellular microenvironment on microglial responses to inflammatory stimuli.
  • To demonstrate the probe's utility in both in vitro and in vivo models.

Main Methods:

  • Development of CDr20-CO1, a probe activated by UGT1A7C and featuring a bio-orthogonal clickable moiety.
Keywords:
click chemistrylive-cell imagingmicrogliaorganelle visualizationsmall molecule fluorescent probe

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Last Updated: May 19, 2026

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  • Live-cell imaging of microglial lysosomes, phospholipids, and proteins in heterogeneous brain cell cultures.
  • In vivo labeling of microglial targets in live mouse embryos.

    • Main Results:

    • CDr20-CO1 successfully visualized microglial targets in live cells and tissues.
    • Microglia in mixed cultures showed altered lysosomal and phospholipid metabolism compared to isolated cells, influenced by cell-cell contact and secreted factors.
    • The probe demonstrated robust tissue permeability and physiological applicability in vivo.

    Conclusions:

    • CDr20-CO1 is a versatile tool for real-time tracking of microglial organelles and biomolecules.
    • The cellular microenvironment significantly modulates microglial inflammatory responses.
    • This platform offers new insights into microglial function in health and disease.