Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Electric Double Layer Enabled Pressure and Position Sensing for Autonomous Underwater Object Grasping.

ACS applied materials & interfaces·2026
Same author

Methods of Soft PDMS Microlens Arrays Fabrication via Air-Expansion-Induced Molding with 3D-Printed Templates.

Small methods·2025
Same author

Electroosmotic Flow of Shear-Thinning Xanthan Gum Solutions in a Rectangular Microchannel.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

TPU/MOFs Electrospun Composite Film for Underwater Tactile Sensing and Finger Joint Bending Monitoring.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

AC Insulator-Based Dielectrophoretic Separation of Live and Dead Yeast Cells.

Electrophoresis·2025
Same author

Polymer solution flow transitions and scaling laws for changing contraction ratios in planar constriction microchannels.

Soft matter·2025

Related Experiment Video

Updated: May 19, 2026

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
10:28

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids

Published on: January 3, 2014

Particle Shape-Dependent Electrophoresis in Viscoelastic Fluids.

Joseph Bentor1, Seyed Mojtaba Tabarhoseini1, Yongxin Song2

  • 1Department of Mechanical Engineering, Clemson University, Clemson, South Carolina 29634-0921, United States.

Analytical Chemistry
|May 18, 2026
PubMed
Summary
This summary is machine-generated.

Fluid elasticity, not particle size, dictates electrophoretic velocity in viscoelastic solutions. Particle shape significantly impacts velocity in non-Newtonian fluids, unlike in Newtonian fluids.

More Related Videos

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions
11:38

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions

Published on: April 19, 2018

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer
08:47

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer

Published on: April 21, 2022

Related Experiment Videos

Last Updated: May 19, 2026

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
10:28

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids

Published on: January 3, 2014

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions
11:38

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions

Published on: April 19, 2018

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer
08:47

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer

Published on: April 21, 2022

Area of Science:

  • Physics
  • Chemistry
  • Biotechnology

Background:

  • Electrophoretic velocity is typically independent of particle size and shape in Newtonian fluids.
  • Previous work identified particle size-dependent electrophoresis in viscoelastic poly(ethylene oxide) solutions.
  • Fluid elasticity's role in particle shape-dependent electrophoresis requires further investigation.

Purpose of the Study:

  • To investigate the influence of fluid elasticity on particle shape-dependent electrophoresis.
  • To determine if particle shape affects electrophoretic velocity in viscoelastic fluids.
  • To explore potential applications in microfluidic separation technologies.

Main Methods:

  • Experimental measurements of electrophoretic velocity for particles of varying shapes (sphere, pear, peanut) in poly(ethylene oxide) solutions.
  • Comparison of results in viscoelastic fluids versus Newtonian fluids.
  • Systematic variation of polymer concentration to assess the effect of fluid elasticity.

Main Results:

  • Electrophoretic velocity is dependent on particle shape in viscoelastic fluids, unlike in Newtonian fluids.
  • Slenderer particle shapes (pear, peanut) exhibit enhanced electrophoretic velocity compared to spherical shapes.
  • This shape dependence intensifies with increasing poly(ethylene oxide) concentration due to heightened fluid elasticity.

Conclusions:

  • Fluid elasticity, rather than particle size alone, induces particle shape dependence in electrophoresis.
  • The observed phenomenon offers a mechanism for label-free electrophoretic separation of particles and cells.
  • Non-Newtonian microfluidic devices can leverage this effect for advanced separation applications.