Effect of food intake on biomarkers measured by the Proseek Multiplex Metabolism panel

Food intake induces metabolic and hemodynamic changes, but its impact on many circulating protein biomarkers remains unclear. This study assessed the effect of a standardised meal on 85 plasma biomarkers measured by the Proseek Multiplex Metabolism panel. Twenty-two healthy adults (11 men, 11 women; mean age 25.9  ±  4.2 years) were examined after an overnight fast and at 30 and 120 min following a standardised meal. Plasma samples were analysed with the Proseek Multiplex Metabolism panel. Twelve biomarkers decreased significantly after food intake; none increased. Eight biomarkers were reduced at 30 min, and four remained decreased at 120 min. The largest changes (>20%) were observed for DNA-(apurinic/apyrimidinic site) endonuclease, eosinophil cationic protein, thyrotropin subunit beta, carbonic anhydrase 13, and Crk-like protein. Food intake has modest effects on most biomarkers in the Proseek Multiplex Metabolism panel, suggesting fasting may not be critical for routine use. However, caution is warranted for biomarkers showing >20% change. Further studies in diverse populations and disease states are needed to confirm these findings.