Biological and toxicological evaluation of chicken eggshell membrane for guided bone regeneration: An in vitro...

The lack of cost-effective, biocompatible and sustainable barrier membranes remains a significant limitation in guided bone regeneration (GBR). Therefore, it is of interest to evaluate the biological compatibility and toxicological safety of chicken eggshell membrane as a potential GBR barrier membrane using standardized in vitro assays. Physical characterization, cell viability, proliferation, cytotoxicity grading and toxicological assessments were performed under controlled laboratory conditions. The membrane demonstrated high cell viability (>94%), progressive proliferation, non-cytotoxic classification and absence of inflammatory or necrotic responses. Thus, we show that chicken eggshell membrane exhibits excellent biocompatibility with minimal toxicological risk, supporting its potential as a sustainable alternative membrane for GBR applications.