A retrospective comparison of CMV DNA detection in reproductive tract secretions versus urine: increased yield with combined sample testing
Related Experiment Video
Updated: May 19, 2026
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Lingwei Hu1, Yongying Bai2, Peihao Wu2
1Department of Genetics and Metabolism, The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China.
Background:
This study aims to compare cytomegalovirus (CMV) DNA detection rates in reproductive tract secretions (RTS) and urine samples and to further evaluate whether multiple specimen testing improves overall CMV DNA detection rates.
Methods:
We conducted a retrospective cohort study involving 3,804 female participants who provided 4,383 samples (10.95% RTS; 89.05% urine) between January 2020 and June 2024. Analyses included: (1) CMV DNA detection rates across sample types and population subgroups, (2) the impact of single versus multiple specimen testing on CMV DNA detection rates.
Result:
CMV DNA was detected in 3.03% (133/4383) of all samples. Positivity rates differed significantly: 9.79% (47/480) for RTS versus 2.20% (86/3903) for urine (p < 0.001). Single-sample testing yielded a positivity rate of 2.46% (82/3,330), while testing multiple specimens increased this to 5.91% (28/474). Concurrent testing of both urine and RTS yielded the highest positivity rate (15.63%, 20/128). Additionally, median CMV viral load was significantly higher in RTS compared to urine (3.34 ± 1.43 vs. 0.81 ± 1.47, p < 0.001).
Conclusions:
The detection rate of CMV DNA in RTS is higher than that in urine. The combined sample testing increases the detection rate of CMV DNA, potentially helping identify women with active viral shedding. These findings inform the development of optimized CMV DNA testing protocols.
