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Updated: May 19, 2026
A Modified Technique for Inducing Polycystic Ovary Syndrome in Mice
Published on: July 5, 2024
Lobetyolin reshapes gut microbiota and bile acid metabolism to improve androgen-driven PCOS phenotypes in mice
Lijuan Li1,2, Pingping Lin2, Siyuan Liu2
1Shanxi Provincial Department-Municipal Key Laboratory Cultivation Base for Quality Enhancement and Utilization of Shangdang Chinese Medicinal Materials, Changzhi Medical College, Changzhi, Shanxi, China.
Aim:
To clarify the molecular mechanisms through which lobetyolin (LT) modifies the gut microbiota-bile acid axis to multitarget the regulation of hyperandrogenism, insulin resistance, chronic low-grade inflammation, and endometrial dysfunction, consequently breaking the vicious cycle and improving the pathological phenotype of polycystic ovary syndrome (PCOS), thereby establishing a theoretical basis and experimental validation for the advancement of LT as a systemic therapeutic agent for PCOS.
Methods:
A DHEA-induced PCOS murine model was administered LT (i.p. for 28 days). We monitored hormone levels in the serum, glucose tolerance, and estrus cycle. Ovarian steroidogenic enzyme expression (CYP11A1, CYP17A1, and CYP19A1) was assessed by qPCR, while inflammatory markers (IL-6, TNF-α, TLR4, and NF-κB) were quantified by ELISA.16S rRNA sequencing was performed for gut microbiota and focused bile acid metabolomics. Uterine molecular markers were evaluated through vascular endothelial growth factor A (VEGFA)/vascular endothelial growth factor receptor (VEGFR2), tissue inhibitor of metalloproteinases 1(TIMP1/2), and matrix metalloproteinase 2/9 (MMP2/9).
Main Results:
LT restored estrous cyclicity, lowered the LH/FSH ratio and serum testosterone levels, and improved insulin sensitivity. Androgen Network Modulation: LT decreased CYP11A1/CYP17A1 and elevated CYP19A1, showing "upstream inhibition-downstream activation." LT also boosted α-diversity, adjusted the F/B ratio, enriched Dubosiella/Muribaculum, and inhibited Lachnospiraceae and Alistipes. Higher HCA and TCDCA levels are associated with improved metabolism. LT alleviated pathological vascular remodeling in the uterus by downregulating VEGFA/VEGFR2 and MMP2/9, and by overexpressing TIMP1/2.
Conclusion:
LT modulates the microbiota-bile acid axis to coordinately ameliorate hyperandrogenism, insulin resistance, chronic inflammation, and uterine dysfunction. This intervention blocks the pathological cascade of PCOS. The findings provide a subsequent mechanistic studies on PCOS prevention.
