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Updated: May 19, 2026

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

ANYI: The ANnotated Yeast Interactome.

Daniel A Nissley^1,2, Muskan Goel³, Xavier Castellanos-Girouard⁴

¹National Synthesis Center for Emergence in the Molecular and Cellular Sciences, National Science Foundation, USA.

Biorxiv : the Preprint Server for Biology

|May 18, 2026

Summary

The ANnotated Yeast Interactome (ANYI) database integrates yeast protein-protein interactions with diverse biological features. This resource enables cross-layer analyses of yeast proteome networks and protein properties.

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Last Updated: May 19, 2026

mRNA Interactome Capture from Plant Protoplasts

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Published on: July 28, 2017

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay

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Published on: December 29, 2023

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Area of Science:

* Molecular and Systems Biology
* Bioinformatics and Computational Biology

Background:

* Existing protein-protein interaction (PPI) databases lack unified network topology and detailed biological annotations for yeast.
* There is a need for a comprehensive, protein-centric resource integrating large-scale network data with biophysical and regulatory information.

Purpose of the Study:

* To develop the ANnotated Yeast Interactome (ANYI), an integrated resource for yeast PPI data.
* To combine network topology with extensive annotations (e.g., protein abundance, half-life, stability, PTMs) in a unified framework.
* To facilitate systematic cross-layer analyses of the yeast interactome.

Main Methods:

* Integrated experimental yeast PPI data with sixteen types of feature annotations.
* Developed a unified matrix of 3,927 proteins and 155 annotation features.
* Created an interactive network browser for data visualization and accessibility.

Main Results:

* ANYI successfully integrates diverse yeast PPI and protein feature data.
* The resource enables systematic investigation of relationships between network properties and protein characteristics.
* Users can analyze correlations between protein connectivity and features like stability or disorder.

Conclusions:

* ANYI provides a valuable, open-access resource for yeast interactome research.
* The integrated framework facilitates novel discoveries in yeast systems biology.
* ANYI empowers computational scientists to explore complex protein interaction networks and associated features.