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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Updated: May 19, 2026

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
10:57

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Published on: October 24, 2019

Modeling human B cell development with pluripotent stem cells.

Xiaoning Sun1,2, Jamie J Kwan1, Krishna Kothari3

  • 1McEwen Stem Cell Institute, University Health Network, Toronto, ON M5G1L7, Canada.

Biorxiv : the Preprint Server for Biology
|May 18, 2026
PubMed
Summary
This summary is machine-generated.

Scientists developed a new method to generate functional B cells from human pluripotent stem cells (hPSCs). This breakthrough offers a promising source for B cell therapies and studying B cell development.

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Published on: June 16, 2019

Area of Science:

  • Stem Cell Biology
  • Immunology
  • Hematopoiesis

Background:

  • Generating functional B cells from human pluripotent stem cells (hPSCs) is crucial for developing novel B cell-based therapies.
  • Existing methods require optimization for efficient and reliable B cell development from hPSCs.

Purpose of the Study:

  • To establish an efficient protocol for developing B lineage cells from hPSC-derived definitive hematopoietic progenitors.
  • To characterize the developmental stages and functional capabilities of hPSC-derived B cells.
  • To investigate signaling pathways regulating B lymphopoiesis and identify potential therapeutic targets.

Main Methods:

  • Generation of definitive hematopoietic progenitors from various hPSC lines.
  • Flow cytometry and multi-omic single-cell RNA sequencing (scRNA-seq) for detailed cellular analysis.
  • Induction of B cell maturation into antibody-secreting plasma cells.

Main Results:

  • The protocol efficiently generated B cells from hPSCs, recapitulating pro-B, pre-B, and naïve B cell stages.
  • hPSC-derived naïve B cells matured into antibody-secreting plasma cells with class-switching capabilities.
  • Interleukin-7 (IL-7) was found to inhibit immunoglobulin heavy chain (IgH) rearrangement, impairing pre-B cell maturation.

Conclusions:

  • This study demonstrates the efficient generation of functional B cells from hPSCs, providing a valuable system for research.
  • The findings offer a source of staged B cells for therapeutic applications and for studying early human B lymphopoiesis.
  • Distinct developmental sources, including definitive and yolk sac progenitors, contribute to human B cell lineage development.