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Oxford Nanopore Technologies RNA sequencing data for investigating epigenetic regulation by phase-variable DNA

Alice Ascari1, Luke V Blakeway1, Calum J Walsh2

  • 1Institute for Biomedicine and Glycomics, Griffith University, Gold Coast, Queensland, Australia.

Microbiology Resource Announcements
|May 18, 2026
PubMed

Insights

Neisseria meningitidis causes meningitis and septicemia globally. This study used RNA sequencing to analyze strain M0579, revealing the epigenetic regulator ModD1 in hyperinvasive meningococcal B lineages.

Area of Science:

  • Microbiology
  • Genomics
  • Epigenetics

Background:

  • Neisseria meningitidis is a major cause of bacterial meningitis and septicemia.
  • This bacterium poses a significant global public health threat.
  • Serogroup B strains are particularly concerning due to their invasive potential.

Purpose of the Study:

  • To perform RNA sequencing on Neisseria meningitidis serogroup B strain M0579.
  • To investigate the role of epigenetic regulators in hyperinvasive meningococcal strains.
  • To characterize the expression profile of the ModD1 gene.

Main Methods:

  • Oxford Nanopore Technologies RNA sequencing was employed.
  • Analysis focused on Neisseria meningitidis serogroup B strain M0579.
  • The study specifically examined the DNA methyltransferase ModD1.

Main Results:

  • The RNA sequencing data provided a comprehensive transcriptomic profile of the strain.
  • The presence and potential function of the phase-variable DNA methyltransferase ModD1 were confirmed.
  • ModD1 was identified as an epigenetic regulator potentially linked to hyperinvasiveness.

Conclusions:

  • The study provides valuable transcriptomic data for Neisseria meningitidis serogroup B.
  • The findings highlight the potential role of ModD1 in the pathogenesis of invasive meningococcal disease.
  • Further research into ModD1 could offer new therapeutic targets.
Keywords:
DNA methylationDNA methyltransferaseModNeisseria meningitidisepigenetic regulationgene regulationpathogenic Neisseriaphase variation

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