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Related Experiment Video

Updated: May 19, 2026

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Paper-based colorimetric test strip for arsenic detection in vegetable oils.

Zhipeng Cai1, Liuping Zhang1,2, Jiaxun Li1

  • 1International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi 214100, Jiangsu, P. R. China. zhanglp0007@163.com.

Analytical Methods : Advancing Methods and Applications
|May 18, 2026
PubMed
Summary

A new paper-based test strip offers rapid, low-cost detection of inorganic arsenic in vegetable oils. This method provides accurate results comparable to expensive techniques, enhancing food safety.

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Area of Science:

  • Analytical Chemistry
  • Food Safety
  • Environmental Science

Background:

  • Arsenic contamination in vegetable oils poses significant human health risks due to its carcinogenic properties.
  • Existing arsenic detection methods are often costly, complex, or struggle with interference from high-lipid oil matrices.

Purpose of the Study:

  • To develop a rapid, cost-effective, and sensitive paper-based colorimetric test strip for quantifying inorganic arsenic in vegetable oils.
  • To overcome the limitations of current analytical techniques for on-site arsenic detection in food matrices.

Main Methods:

  • Modification of the classic Gutzeit method for a paper substrate.
  • Optimization of paper substrate, mercuric bromide concentration, and dithiothreitol-assisted pretreatment.
  • Development of a quantitative colorimetric assay for inorganic arsenic detection.

Main Results:

  • Achieved a limit of detection of 0.05 mg kg⁻¹ with a linear range of 0.05-1 mg kg⁻¹.
  • Completed the entire analysis process within 30 minutes.
  • Demonstrated high consistency with ICP-MS, showing recoveries between 94.13-109.12% in spiked and real samples.
  • Confirmed reliable performance after 28 days of storage under various temperature conditions.

Conclusions:

  • The developed paper-based test strip is an efficient, low-cost, on-site alternative for arsenic detection in vegetable oils.
  • This method addresses the need for rapid grassroots screening of arsenic contamination in food products.
  • The test strip offers a practical solution for enhancing food safety and mitigating dietary health risks associated with arsenic exposure.