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Related Experiment Video

Updated: May 19, 2026

Using R, Seurat, and CellChat to Analyze a Single-Cell Transcriptomics Dataset of Mouse Skin Wound Healing
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Published on: August 1, 2025

Macrophage-Fibroblast Crosstalk Shapes Wound Repair Signaling In Vitro.

Daniela Enriquez-Ochoa1,2, Raji R Nagalla1,2, Yingzi Liu3,4

  • 1Department of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|May 18, 2026
PubMed
Summary
This summary is machine-generated.

Macrophages and fibroblasts coordinate wound healing through direct contact and secreted signals. This study reveals how cell-cell communication, including calcium and connexin signaling, shapes their behavior during tissue repair.

Keywords:
calcium signalingcell–cell contactconnexin 43fibroblastsmacrophagessingle‐cell RNA sequencingwound healing

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Published on: March 25, 2020

Area of Science:

  • Cell Biology
  • Immunology
  • Tissue Engineering

Background:

  • Wound healing involves complex interactions between immune cells like macrophages and structural cells like fibroblasts.
  • The integration of contact-dependent and paracrine signaling in macrophage-fibroblast communication during healing remains incompletely understood.

Purpose of the Study:

  • To investigate the mechanisms of macrophage-fibroblast communication during in vitro wound healing.
  • To elucidate how direct cell contact and paracrine factors influence reciprocal cell behaviors and signaling pathways.

Main Methods:

  • Utilized complementary 2D and 3D in vitro wound healing models.
  • Employed live-cell calcium imaging and single-cell RNA sequencing (scRNA-seq).
  • Investigated the roles of connexins and cell adhesion molecules (CAMs).

Main Results:

  • Macrophage contact with fibroblasts promoted scratch closure independently of connexins.
  • Fibroblast contact enhanced macrophage IL10 production via connexin 43 (Cx43)-dependent signaling and suppressed TNFα secretion through paracrine mechanisms.
  • Fibroblast-macrophage contact induced connexin-dependent calcium signals in macrophages, and scRNA-seq revealed dynamic regulation of CAMs and signaling pathways post-wounding.

Conclusions:

  • Macrophage-fibroblast communication integrates contact-dependent calcium/connexin signaling with transcriptional changes.
  • This coordinated signaling network is crucial for regulating cell behavior during wound healing.
  • Findings provide insights into the cellular and molecular basis of tissue repair processes.