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BMSC-Derived Exosomal miR-874-3p Protects against OGD/R-Induced Neuronal Injury in PC12 Cells via Regulating KPNA4
Guangming Dai1, Yuhang Li2, Bo Feng3
1Department of Hand, Foot and Ankle Surgery, The Third Affiliated Hospital of Inner Mongolia Medical University, No.20, Shaoxian Road, Kundulun District, Baotou, 014010, Inner Mongolia Autonomous Region, P. R. China.
Neurochemical Research
|May 18, 2026
Summary
Exosomes carrying miR-874-3p from bone marrow mesenchymal stem cells (BMSCs) protect against sciatic nerve injury by reducing apoptosis and inflammation. This therapeutic effect is achieved by targeting KPNA4, offering a novel treatment strategy.
Area of Science:
- Biomedical Science
- Regenerative Medicine
- Neuroscience
Background:
- Sciatic nerve injury is a common peripheral nerve injury with significant patient impact.
- MicroRNA-874-3p (miR-874-3p) is known to influence cell proliferation, immune response, and apoptosis.
- The precise role of miR-874-3p in sciatic nerve injury is not well understood.
Purpose of the Study:
- To investigate the therapeutic potential of exosomal miR-874-3p derived from bone marrow mesenchymal stem cells (BMSCs) in sciatic nerve injury.
- To elucidate the underlying molecular mechanisms of exosomal miR-874-3p in mitigating neuronal damage.
Main Methods:
- Bone marrow mesenchymal stem cells (BMSCs) were isolated and exosomes were extracted.
- Cell viability, apoptosis, oxidative stress markers (ROS, MDA, SOD, GSH-Px), and inflammatory factors were assessed.
- Gene and protein expression were analyzed using qPCR and western blot, with dual-luciferase reporter assay to confirm target interaction.
Main Results:
- BMSCs-derived exosomes showed upregulated miR-874-3p.
- Exosomal miR-874-3p supplementation enhanced cell viability and reduced apoptosis.
- It also modulated oxidative stress and suppressed inflammatory factors, thereby decelerating neuronal impairment.
- These effects were mediated by the inhibition of KPNA4, as KPNA4 overexpression reversed the protective effects.
Conclusions:
- Exosomal miR-874-3p from BMSCs effectively alleviates OGD/R-induced neuronal injury.
- The therapeutic mechanism involves targeting KPNA4.
- This highlights exosomal miR-874-3p as a promising therapeutic agent for sciatic nerve injury.
