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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

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Related Experiment Video

Updated: May 20, 2026

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
09:13

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

Published on: November 11, 2010

The interconnection of cryobiology and tissue engineering.

Vitalii Mutsenko1, Lilia Kuleshova2, Birgit Glasmacher3

  • 1Institute for Multiphase Processes, Leibniz University Hannover, Garbsen, Germany; Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine.

Cryobiology
|May 18, 2026
PubMed
Summary

Cryobiology is revolutionizing tissue engineering by enabling the preservation and clinical translation of tissue-engineered constructs (TECs). Advances in vitrification, novel cryoprotective systems, and cryobioprinting are key to developing off-the-shelf TECs.

Keywords:
BiomaterialsCryobiologyCryobioprinitngCryopreservationSlow freezingTissue engineeringTissue-engineered constructsVitrification

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Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells
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Last Updated: May 20, 2026

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
09:13

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

Published on: November 11, 2010

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells
04:10

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Published on: October 20, 2023

Area of Science:

  • Cryobiology and Tissue Engineering

Background:

  • Cryobiology is increasingly vital for advancing tissue-engineered constructs (TECs).
  • This review focuses on recent breakthroughs at the intersection of cryobiology and tissue engineering.

Purpose of the Study:

  • To provide a comprehensive overview of recent advances in cryobiology for tissue engineering.
  • To discuss methods for preserving TECs and their clinical translation.

Main Methods:

  • Review of vitrification techniques for scaffold-based and scaffold-free TECs.
  • Examination of controlled-rate slow-cooling/freezing protocols with xeno-free and DMSO-free systems.
  • Discussion of cryobioprinting and synergistic approaches for enhanced cryopreservation.

Main Results:

  • Vitrification and upscaling methods for TECs, including organoids, are detailed.
  • Xeno-free and DMSO-free cryoprotective systems show promise for cell viability.
  • Preclinical studies demonstrate the potential of cryopreserved TECs for clinical translation.

Conclusions:

  • Cryobiology plays a transformative role in tissue engineering advances.
  • Integrating cryobiology, tissue engineering, and transplantation science is crucial for scalable, off-the-shelf TECs.
  • Novel strategies like cryobioprinting offer new avenues for TEC fabrication and deployment.