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Related Experiment Videos

Mouse immunoglobulin heavy chains are coded by multiple germ line variable region genes.

P Barstad, V Farnsworth, M Weigert

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

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    Mouse immunoglobulin heavy chains show significant sequence diversity, suggesting at least eight germ line genes. This variation in antibody structure correlates with different binding specificities for haptens.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Immunoglobulins (antibodies) are crucial for adaptive immunity.
    • The variable region of immunoglobulin heavy chains determines antigen-binding specificity.
    • Myeloma proteins provide a source for studying immunoglobulin structure and diversity.

    Purpose of the Study:

    • To analyze the sequence diversity of the N-terminal 20 residues of mouse immunoglobulin heavy chains.
    • To infer the number of germ line genes encoding these variable regions.
    • To correlate sequence variation with hapten-binding specificities.

    Main Methods:

    • Comparative sequence analysis of N-terminal 20 residues from 13 BALB/c mouse myeloma heavy chains.
    • Comparison with 15 heavy chains from existing literature.

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  • Analysis of sequence patterns and clustering into sets and subsets.

    • Main Results:

    • Sixteen of 28 heavy chain sequences exhibited differences.
    • Proteins clustered into four major sets, with the largest set further divided into at least five subsets.
    • Sequence diversity suggests a minimum of eight germ line genes for mouse heavy chain variable regions.
    • Distinct amino acid sequences correlated with differing hapten-binding specificities.

    Conclusions:

    • The variable region of mouse immunoglobulin heavy chains is highly diverse.
    • This diversity is likely encoded by at least eight germ line genes.
    • Specific amino acid sequences within the heavy chain variable region are critical for determining hapten specificity.