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Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Studying the Cytoskeleton01:17

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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Extrinsic and Intrinsic Pathways of Hemostasis

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  3. Structural Studies Of Core Hippo Pathway Components.
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  3. Structural Studies Of Core Hippo Pathway Components.

Related Experiment Video

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor

Published on: September 13, 2018

Structural Studies of Core Hippo Pathway Components.

Meihua Lu1,2, Xuelian Luo3,2

  • 1Westlake Laboratory of Life Sciences and Biomedicine, Westlake University, Hangzhou 310024, China.

Cold Spring Harbor Perspectives in Biology
|May 18, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Structural biology reveals the Hippo pathway

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Cell Signaling

Background:

  • The Hippo signaling pathway is crucial for organ size control, tissue homeostasis, and tumor suppression.
  • It regulates key transcriptional coactivators, Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ).
  • Understanding its molecular mechanisms is vital for therapeutic applications.

Purpose of the Study:

  • To review recent structural biology advances in the Hippo signaling pathway.
  • To elucidate the molecular architecture and regulatory mechanisms of core signaling complexes.
  • To provide insights into disease-associated mutations and therapeutic potential.

Main Methods:

  • Review of structural biology studies on Hippo pathway components.
  • Analysis of core signaling complexes: MST1/2-SAV1 and LATS1/2-MOB1.
  • Examination of regulatory complexes like STRIPAK and upstream proteins (NF2/Merlin, angiomotin).

    • Main Results:

    • Detailed insights into the assembly and activation dynamics of MST1/2-SAV1 and LATS1/2-MOB1 complexes.
    • The STRIPAK complex identified as a key negative regulator via its PP2A-striatin core.
    • Angiomotin proteins' role in pathway activation through conformational changes and spatial organization.
    • Disease mutations identified at critical structural interfaces, explaining dysregulation.

    Conclusions:

    • Structural insights significantly advance the understanding of Hippo pathway signal transduction.
    • These findings provide a foundation for developing regenerative medicine and cancer therapies.
    • Targeting structural interfaces offers potential therapeutic strategies.