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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: May 20, 2026

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
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Integrated Transcriptomic and Functional Analyses Reveal lncRNA-miRNA-mRNA Axis in AML Relapse After Allo-HSCT.

Fei Zhao1,2, Wenhao Wang3, Hongye Gao1,2

  • 1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Hematopoietic Stem Cell Transplantation Center, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, P. R. China.

Cancer Science
|May 18, 2026
PubMed
Summary
This summary is machine-generated.

Long non-coding RNAs (lncRNAs) drive relapse in acute myeloid leukemia (AML) after allogeneic stem cell transplant. Targeting the SNHG8 lncRNA axis offers a potential therapeutic strategy for AML relapse.

Keywords:
acute myeloid leukemia (AML)allogeneic hematopoietic stem cell transplantation (Allo‐HSCT) relapseceRNA networkshypomethylating agents (HMA)long non‐coding RNA (lncRNA)

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Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Relapse post-allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a major challenge in acute myeloid leukemia (AML) treatment.
  • Mechanisms of non-coding RNA regulation in AML relapse clones are not fully understood.

Purpose of the Study:

  • To investigate the role of long non-coding RNAs (lncRNAs) in AML relapse after allo-HSCT.
  • To construct competing endogenous RNA (ceRNA) networks to identify regulatory axes involved in relapse.

Main Methods:

  • Transcriptomic profiling of CD34+ cells from relapsed and remitted AML patients post-allo-HSCT.
  • Construction of relapse-associated ceRNA networks using correlation analysis, network topology, and thermodynamic modeling.
  • Functional validation using CRISPR-based xenograft assays and single-cell CRISPR screening (Perturb-seq-like strategy).

Main Results:

  • lncRNAs showed the largest differential expression in relapsed AML post-allo-HSCT.
  • Identified lncRNA-miRNA-mRNA axes potentially driving leukemic progression.
  • Discovered SNHG8 as a key regulator promoting relapse via the SNHG8-miR-625-ZC3H13/15 axis; SNHG8 knockdown inhibited AML growth and induced apoptosis.
  • Hypomethylating agents (HMAs) differentially modulated lncRNA/ceRNA signatures.

Conclusions:

  • lncRNA-ceRNA networks are implicated in post-transplant AML relapse.
  • The SNHG8 axis represents a potential therapeutic vulnerability in AML relapse.
  • Findings support further investigation of lncRNA-targeted therapies for AML relapse post-allo-HSCT.