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Updated: May 20, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

A high-throughput modular microfluidic platform for versatile functional assays at single-cell level.

Ning Shao¹, Junhua Mai², Biana Godin²

¹Center for BioNanoengineering, Houston Methodist Research Institute, Houston, TX, USA. shaoning064@gmail.com.

Microsystems & Nanoengineering

|May 18, 2026

Summary

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HiSCOPE, a new microfluidic platform, analyzes the functional dynamics of thousands of single cells, revealing hidden cellular diversity. This technology aids in understanding complex biology, disease, and advancing personalized medicine.

Area of Science:

Single-cell biology
Microfluidics
Functional genomics

Background:

Current single-cell technologies offer limited functional insights, providing only static molecular data.
Understanding dynamic cellular behavior is crucial for deciphering biological complexity and disease mechanisms.

Purpose of the Study:

To introduce the High-throughput Single-Cell Omni-functional Profiling Engine (HiSCOPE) for dynamic single-cell functional profiling.
To demonstrate HiSCOPE's capability in analyzing diverse cellular functions and interactions.

Main Methods:

Development of a microfluidic platform (HiSCOPE) with hydrodynamic trapping for single cells, pairs, and trios.
Integration of modular assay chambers to support various functional assays (viability, proliferation, cytotoxicity, cytokine secretion, cell-cell interactions).

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Last Updated: May 20, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

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A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

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Incorporation of live-cell retrieval for downstream molecular analysis.

Main Results:

HiSCOPE successfully profiled functional dynamics of over 10,000 single cells.
Demonstrated utility in evaluating drug susceptibility in leukemia cells and NK cell cytotoxicity.
Dissected T cell-macrophage crosstalk, revealing functional diversity masked in bulk assays.

Conclusions:

HiSCOPE provides a scalable solution for high-throughput functional profiling of single cells.
The platform enables deeper understanding of cellular heterogeneity and interactions.
HiSCOPE has broad implications for advancing immunotherapy and personalized medicine.