Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...
Photosystem II01:22

Photosystem II

The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
Oxygenic Photosynthesis01:26

Oxygenic Photosynthesis

Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate light...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early diagnosis of capivasertib-associated severe hyperglycemia with diabetic ketosis diagnosed in routine clinical practice: a case report with review of literature.

Endocrine journal·2026
Same author

Age-Related Differences in Neural Networks for Error Detection and Inhibitory Control: A LORETA-Based Comparative Study.

Brain sciences·2026
Same author

Rational donor placement into a native exciton manifold: cavity-anchored dyes drive sub-picosecond energy transfer in light-harvesting complex 2.

Physical chemistry chemical physics : PCCP·2026
Same author

Measurement Technologies for Ankle-Dorsiflexion Function After Stroke: A Systematic Review and Meta-Analysis of Sensing Approaches and Their Relationships with Gait Performance.

Sensors (Basel, Switzerland)·2026
Same author

Molecular Crystals With Reversible Chromic Three-State Crystal-to-Crystal Transformation via the Dynamic Motion of Negatively Curved π-Frameworks.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Larger balloon diameter improves vascular resistance in arteriovenous access: A retrospective analysis.

The International journal of artificial organs·2026

Related Experiment Video

Updated: May 20, 2026

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
04:11

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

A copper-dependent redox-based hydrogen peroxide perception in plants.

Nobuaki Ishihama1, Yohta Fukuda2,3, Yumiko Shirano4

  • 1RIKEN Center for Sustainable Resource Science, Yokohama, Japan.

Nature Communications
|May 18, 2026
PubMed
Summary

Plant receptor CARD1 distinguishes between quinones and reactive oxygen species (ROS) using a novel copper-dependent mechanism. This finding reveals a unique redox perception pathway in plants crucial for signaling.

More Related Videos

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
09:47

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells

Published on: June 2, 2023

ROS Live Cell Imaging During Neuronal Development
09:25

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
04:11

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
09:47

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells

Published on: June 2, 2023

ROS Live Cell Imaging During Neuronal Development
09:25

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

Area of Science:

  • Plant biology
  • Molecular signaling
  • Biochemistry

Background:

  • Redox-related molecules like quinones and reactive oxygen species (ROS) are vital signaling molecules.
  • Plant leucine-rich repeat receptor-like kinase (LRR-RLK) CARD1 (also known as HPCA1) perceives both quinones and ROS.
  • The mechanism by which CARD1 differentiates between these signals is not well understood.

Purpose of the Study:

  • To elucidate the structural basis of signal perception in the plant LRR-RLK CARD1.
  • To understand how CARD1 distinguishes between quinone and ROS signals.
  • To investigate the role of unique cysteine residues and metal ions in CARD1 signaling.

Main Methods:

  • X-ray crystallography to determine the structure of the CARD1 ectodomain.
  • Genetic analysis to assess the function of specific residues.
  • Biochemical assays to study protein-ligand interactions and signaling mechanisms.

Main Results:

  • The structure of the CARD1 ectodomain revealed unique features.
  • Previously identified unique cysteine residues are not essential for CARD1 signal perception.
  • CARD1 utilizes a surface-adsorbed copper ion, coordinated by histidine residues, for hydrogen peroxide signaling.

Conclusions:

  • CARD1 employs a unique copper-dependent mechanism for perceiving hydrogen peroxide signals.
  • This study uncovers a novel mode of redox perception in plants.
  • The findings provide insights into receptor interactions with non-peptide stimuli.