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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: May 20, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Coordinated alternative splicing decisions via stepwise exon definition.

Panajot Kristofori1,2, Zijie Xiao1,2, Simon Braun3

  • 1Department of Systems Biology, Institute for Biomedical Genetics (IBMG), University of Stuttgart, 70569 Stuttgart, Germany.

Nucleic Acids Research
|May 19, 2026
PubMed
Summary

Alternative splicing, a key gene regulation process, involves coordinated exon inclusion and intron retention. This study reveals how spliceosome assembly dictates these events, uncovering mechanisms disrupted in cancer.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Computational Biology

Background:

  • Alternative splicing of pre-messenger RNA (mRNA) is crucial for gene regulation in humans.
  • Aberrant splicing, including alternative exon (AE) skipping/inclusion and intron retention (IR), is linked to diseases like cancer.
  • The spliceosome orchestrates splicing, but its coordination of diverse splicing decisions remains unclear.

Purpose of the Study:

  • To investigate the coordination between alternative exon inclusion and intron retention.
  • To elucidate the molecular mechanisms underlying the regulation of these splicing events.
  • To explore the role of spliceosome dynamics in cancer-associated splicing deregulation.

Main Methods:

  • Analysis of large-scale mutagenesis screening data.
  • Transcriptome-wide RNA sequencing (RNA-seq) for splicing analysis.
  • Data-driven mathematical modeling of spliceosome assembly and function.

Main Results:

  • Intron retention adjacent to alternative exons frequently occurs at intermediate inclusion levels.
  • Multistep exon recognition, driven by spliceosome assembly, explains the dependency between AE inclusion and IR.
  • This multistep recognition is perturbed in cancer cells, leading to coordinated splicing deregulation.

Conclusions:

  • Stepwise alternative exon recognition by the spliceosome coordinates AE inclusion and flanking intron retention.
  • This mechanism provides insights into how spliceosome dynamics regulate splicing decisions.
  • Understanding these coordinated events may reveal common molecular mechanisms underlying splicing errors in cancer.