The miR-195-3p is a Novel Prognostic Biomarker, Inhibiting Angiogenesis in Lung Adenocarcinoma

In different kinds of tumors, miR-195-3p showed converse roles. How-ever, the role of miR-195-3p in lung adenocarcinoma (LUAD) remains unclear. To explore the prognostic role and potential underlying mechanisms of miR-195-3p in LUAD.

In this study, we analyzed the correlation between the miR-195-3p expression level and clinicopathological features in LUAD based on the TCGA Database. The prognostic role of miR-195-3p was explored, and we then constructed a nomogram. The expression levels of miR-195-3p in LUAD tumor tissues and adjacent normal lung tissues were further determined by qRT-PCR in clinical samples. Finally, we explored the mechanisms by which miR-195-3p affects patient prognosis.

The results showed that miR-195-3p was expressed at decreased levels in LUAD tumor tissue. Furthermore, elevated miR-195-3p expression was associated with a better prognosis and served as an independent prognostic factor in LUAD. CD34 immunohistochemical staining showed that miR-195-3p expression was negatively correlated with microvessel density (MVD). Overexpression of miR-195-3p inhibited the migration and tube formation ability of HUVEC (Human Umbilical Vein Endothelial Cells).

Our findings demonstrate that miR-195-3p expression is significantly downregulated in LUAD tumor tissues compared to normal lung tissues, corroborating its tumor-suppressive role. The experiment suggests that this miRNA may exert its prognostic influence by modulating angiogenesis. However, this study still has several limitations that need to be addressed in future work.

These findings indicate that miR-195-3p is a useful prognostic biomarker in LUAD, as well as a potential therapeutic target.