Related Experiment Video
Updated: May 20, 2026
08:51
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
Published on: September 4, 2012
Changes in Antimicrobial Component Concentrations in Goat Milk Following Intramammary Lipopolysaccharide
Jirapat Jaisue1, Moeka Nishimura1, Naoki Suzuki1
1Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Hiroshima, Japan.
Animal Science Journal = Nihon Chikusan Gakkaiho
|May 19, 2026
Summary
This study shows milk antimicrobial components can stage mastitis progression. Changes in concentrations of these components indicate inflammation or recovery, aiding judicious antimicrobial use in dairy goats.
Area of Science:
- Veterinary Medicine
- Animal Science
- Biochemistry
Background:
- Mastitis significantly impacts dairy production economics, with prevention and treatment challenges.
- Current mastitis management often involves broad antimicrobial use, even when pathogens aren't detected, potentially leading to overuse.
- There's a need for better indicators to distinguish inflammatory and healing phases of mastitis for targeted treatment.
Purpose of the Study:
- To investigate if milk antimicrobial component (AMC) concentrations can indicate the inflammatory stage of mastitis.
- To support judicious antimicrobial use by identifying reliable biomarkers for mastitis progression.
- To differentiate between early inflammation and recovery phases using milk AMC profiles.
Main Methods:
- Experimental mastitis was induced in lactating Tokara goats using intramammary lipopolysaccharide (LPS) infusion.
- Milk samples were collected over 120 hours post-infusion.
- Somatic cell counts (SCC) and concentrations of lactoferrin (LF), goat β-defensin 1 (GBD1), cathelicidin-7, S100A8, and milk amyloid A (MAA) were measured.
Main Results:
- Somatic cell counts (SCC) peaked at 10 hours post-infusion.
- Goat β-defensin 1 (GBD1) peaked early at 4 hours, indicating initial inflammation.
- Lactoferrin (LF), S100A8, and milk amyloid A (MAA) peaked later (36-84 hours) as SCC decreased, correlating with the recovery phase.
Conclusions:
- Temporal patterns of milk AMCs can effectively stage mastitis progression in goats.
- Specific AMCs show distinct temporal dynamics corresponding to inflammation and recovery.
- These findings facilitate more precise management of mastitis, optimizing antimicrobial therapy.
More Related Videos
Related Concept Videos
01:25Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
01:19Formation of Lipopolysaccharides
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
01:18Surface Membrane Barriers
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:23Inhibitors of Gram-positive Cell Wall Synthesis
Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...