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Updated: May 20, 2026

Rapid Isolation of Dorsal Root Ganglion Macrophages

Rapid Isolation of Dorsal Root Ganglion Macrophages

Published on: September 7, 2019

High-dimensional Spatial Immune Profiling Highlights Microglia-Like Cells in Human Dorsal Root Ganglia.

Marius Schwabenland¹, Busranur Oeztuerk¹, Thomas Blank¹

¹Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

European Journal of Immunology

|May 19, 2026

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Immunity·2026

Human dorsal root ganglia (DRG) contain myeloid cells with microglial features, spatially mapped using Imaging Mass Cytometry. These findings reveal distinct neuroimmune niches in the peripheral nervous system relevant to pain.

Area of Science:

Neuroscience
Immunology
Cell Biology

Background:

Human dorsal root ganglia (DRG) are recognized as immune-active sites.
Previous studies identified microglia-like myeloid cells in DRG using transcriptomics, but protein-level spatial data in humans was missing.

Purpose of the Study:

To create a spatially resolved, protein-level atlas of myeloid cells in human DRG.
To characterize myeloid cell subsets and their localization relative to neurons in situ.

Main Methods:

Utilized highly multiplexed Imaging Mass Cytometry (IMC) with a 41-marker panel.
Performed subcellular resolution mapping and phenotyping of immune and neural cells in human DRG.
Applied unsupervised clustering (FlowSOM) to >6000 Iba1+ cells.

Keywords:

dorsal root ganglia imaging mass cytometry microglia, microglia‐like cells myeloid cells spatial immune profiling

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Last Updated: May 20, 2026

Rapid Isolation of Dorsal Root Ganglion Macrophages

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Published on: September 7, 2019

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice

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Published on: August 7, 2020

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

Main Results:

Identified Iba1+ myeloid cells co-expressing microglial markers (P2RY12, TMEM119, SLC2A5) near neuronal somata.
Clustering revealed eight distinct myeloid cell subsets, including those with microglial signatures near neurofilament+ neurons.
Distinct myeloid subsets expressing activation markers (CD68, HLA-DR) were spatially segregated from microglial-like cells.

Conclusions:

Provides a detailed, spatially resolved protein-level atlas of myeloid subsets in human DRG.
Characterizes distinct neuroimmune niches within the DRG.
Offers a resource for studying neuroimmune interactions in chronic pain and peripheral neuropathies.