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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: May 20, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
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Published on: May 27, 2016

A Split sfGFP-Based Signal-Amplified Reporter System for Enhanced Detection of Promoter Activity.

Shuo Lin1, Hui Yuan1, Ziyi Lin1

  • 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.

Biotechnology Journal
|May 19, 2026
PubMed
Summary

Researchers developed a sensitive fluorescent reporter system to visualize low-abundance proteins in real-time. This novel split superfolder green fluorescent protein (sfGFP) system enhances signals for improved gene expression analysis in living cells.

Keywords:
coiled‐coil dimerizationpromoter activitysignal‐amplified reportersplit sfGFPtandem

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • Fluorescent reporters offer real-time, single-cell gene expression insights.
  • Current systems lack sensitivity for detecting low-abundance proteins.

Purpose of the Study:

  • To develop a highly sensitive fluorescent reporter system for visualizing low-abundance proteins.
  • To enhance signal amplification for improved detection of weak gene expression.

Main Methods:

  • Developed a split superfolder green fluorescent protein (sfGFP) reporter system.
  • Incorporated a 20x tandem GFP11 tag and coiled-coil dimerization domains.
  • Utilized constitutive and weak promoters for fragment expression.

Main Results:

  • Achieved approximately 10-fold signal enhancement compared to conventional reporters.
  • Enabled clear visualization of fluorescence at extremely low mRNA levels.
  • Identified significant basal leakage in Tet-On 2G systems and enhanced monitoring of endogenous weak promoters.

Conclusions:

  • The developed sfGFP reporter system offers high sensitivity and versatility.
  • Enables accurate analysis of weak promoter activity and dynamic tracking of low-abundance genes.
  • Provides a valuable tool for investigating cell types and states based on gene expression.